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13752
SignalSlide® LKB1 IHC Controls
Experimental Controls
IHC Control Slides

SignalSlide® LKB1 IHC Controls #13752

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SignalSlide® LKB1 IHC Controls: Image 1

Immunohistochemical analysis of paraffin-embedded LNCaP (left) and A549 (right) cell pellets using LKB1 (D60C5F10) Rabbit mAb (IHC Formulated) #13031.

To Purchase # 13752S
Product # Size Price
13752S
1 Pack  (5 slides) $ 144

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Important Ordering Details

Custom Ordering Details: Sections are cut freshly upon ordering. Please allow up to three business days for your product to be processed.

Product Description

Each control slide contains formalin fixed, paraffin-embedded cell pellets, LNCaP (LKB1 positive) and A549 (LKB1 negative), that serve as a control for LKB1 immunostaining.

Background

LKB1 (STK11) is a serine/threonine kinase and tumor suppressor that helps control cell structure, apoptosis and energy homeostasis through regulation of numerous downstream kinases (1,2). A cytosolic protein complex comprised of LKB1, putative kinase STRAD, and the MO25 scaffold protein, activates both AMP-activated protein kinase (AMPK) and several AMPK-related kinases (3). AMPK plays a predominant role as the master regulator of cellular energy homeostasis, controlling downstream effectors that regulate cell growth and apoptosis in response to cellular ATP concentrations (4). LKB1 appears to be phosphorylated in cells at several sites, including human LKB1 at Ser31/325/428 and Thr189/336/363 (5).

Mutation in the corresponding LKB1 gene causes Peutz-Jeghers syndrome (PJS), an autosomal dominant disorder characterized by benign GI tract polyps and dark skin lesions of the mouth, hands, and feet (6). A variety of other LKB1 gene mutations have been associated with the formation of sporadic cancers in several tissues (7).

  1. Baas, A.F. et al. (2004) Trends Cell Biol 14, 312-9.
  2. Marignani, P.A. (2005) J Clin Pathol 58, 15-9.
  3. Lizcano, J.M. et al. (2004) EMBO J 23, 833-43.
  4. Hardie, D.G. (2004) J Cell Sci 117, 5479-87.
  5. Sapkota, G.P. et al. (2002) Biochem J 362, 481-90.
  6. Jenne, D.E. et al. (1998) Nat Genet 18, 38-43.
  7. Sanchez-Cespedes, M. (2007) Oncogene 26, 7825-32.

Pathways & Proteins

Explore pathways + proteins related to this product.

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