Render Target: STATIC
Render Timestamp: 2024-11-05T10:55:22.013Z
Commit: 57f6e368eba1a427377652f2ad915d45d7f340a4
XML generation date: 2024-09-20 06:20:10.138
Product last modified at: 2024-10-17T19:00:08.903Z
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PDP - Template Name: Protein Control Kit
PDP - Template ID: *******5c6809e

VEGF Receptor 2 Control Proteins #2904

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  • WB

    Product Information

    Product Usage Information

    Boil for 3 minutes prior to use. Load 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGF Receptor 2 Control Proteins per lane.

    Storage

    Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v phenol red or bromophenol blue. Store at –20°C or at –80°C for long term storage.

    Product Description

    VEGFR2 Control Protein (Nonphosphorylated): Nonphosphorylated human VEGFR2 (Val789-Val1356) fusion protein treated with λ phosphatase serves as a negative control. Supplied in SDS Sample Buffer.
    VEGFR2 Control Protein (Phosphorylated): Phosphorylated human VEGFR2 (Val789-Val1356) fusion protein serves as a positive control. Supplied in SDS Sample Buffer.
    MW (kDa) 110 ( recombinant kinase fragment)

    Background

    Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells in vitro and angiogenesis in vivo (6-8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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