|23428S||1 Kit (100 assays)||
|Product Includes||Quantity (with Count)||Reactivity|
|CD3 (UCHT1) Mouse mAb (PE Conjugate) 46233||1 x 500 µl||H|
|CD4 (RPA-T4) Mouse mAb (violetFluor™ 450 Conjugate) 26755||1 x 500 µl||H|
|CD8α (RPA-T8) Mouse mAb (FITC Conjugate) 55397||1 x 500 µl||H|
|IL2-Rα/CD25 (BC96) Mouse mAb (APC Conjugate) 20164||1 x 500 µl||H|
|CD69 (FN50) Mouse mAb (PE-Cy7® Conjugate) 85313||1 x 500 µl||H|
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Count cells using a hemocytometer or alternative method.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to immunostaining.
NOTE: Human Fc receptors cross-react with rabbit IgG. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubate live cells with human Fc block prior to immunostaining with rabbit antibodies.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
posted June 2017
revised January 2022
Protocol Id: 1504
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
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