|Product Includes||Quantity (with Count)||Reactivity||Dilution|
|CD3 (17A2) Rat mAb (PE Conjugate) 28306||1 x 100 µg||M||1:40|
|CD4 (RM4-5) Rat mAb (violetFluor™ 450 Conjugate) 92599||1 x 100 µg||M||1:160|
|CD8α (2.43) Rat mAb (PE-Cy7® Conjugate) 87922||1 x 100 µg||M||1:80|
|CD44 (IM7) Rat mAb (PE-Cy5® Conjugate) 94170||1 x 100 µg||H M||1:160|
|CD62L/L-Selectin (MEL-14) Rat mAb (FITC Conjugate) 76378||1 x 100 µg||M||1:200|
|CD127/IL-7Rα (A7R34) Rat mAb (APC Conjugate) 28470||1 x 100 µg||M||1:80|
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: When including fluorescent cellular dyes in your experiment (including viability dyes, DNA dyes, etc.), please refer to the dye product page for the recommended protocol. Visit www.cellsignal.com for a full listing of cellular dyes validated for use in flow cytometry.
NOTE: Count cells using a hemocytometer or alternative method.
NOTE: If using whole blood, lyse red blood cells and wash by centrifugation prior to immunostaining.
NOTE: Human Fc receptors cross-react with rabbit IgG. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubate live cells with human Fc block prior to immunostaining with rabbit antibodies.
NOTE: Optimal centrifugation conditions will vary depending upon cell type and reagent volume. Generally, 150-300g for 1-5 minutes will be sufficient to pellet the cells.
posted June 2017
revised January 2022
Protocol Id: 1504
Antibodies are supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. Store at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature. Please refer to product labels, datasheets, or web pages for specific “Best By” dates for each individual component.
Monoclonal antibodies were purified from tissue culture supernatant via affinity chromatography. The purified antibodies were conjugated under optimal conditions, with unreacted dye removed from the preparation.
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