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Neutral Protease (Dispase®)
Flow Cytometry Kits & Reagents

Neutral Protease (Dispase®) #44439

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Product Usage Information

Neutral Protease (Dispase®) is stable over a wide pH range (4.0-9.0) with an optimal pH range of 5.9-7.0. It is recommended to reconstitute with a buffer compatible with the intended assay.


Neutral Protease (Dispase®) is supplied as a lyophilized powder. This product is stable for 12 months when stored at 4ºC, protected from moisture. It is recommended to reconstitute as needed and to store solutions at -20ºC or -80ºC. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Neutral Protease (Dispase®) is isolated from Bacillus polymyxa and is a mild proteolytic enzyme that separates the epidermis from dermis by cleaving the basement membrane zone of the skin while preserving the viability of epithelial sheets (1,2). This enzyme is effective at cleaving fibronectin and type IV collagen in the lamina densa of skin. It will not cleave serum albumin, transferrin, laminin, type V collagen, or anchoring fibrils containing type VII collagen (1). As described in the expired U.S. Patent 4,304,866, Neutral Protease (Dispase®) is activated by metallic ions such as Ca++, Mn++, Mg++, Fe++, Fe+++, and Al+++ and is inhibited by metal-chelating agents such as ethylenediaminetetraacetic acid (EDTA), citric acid, o-phenanthroline, 2,2'-dipyridyl and sodium fluoride, and oxidizing agents such as N-bromosuccinimide (NBS) and iodine (3). Neutral Protease (Dispase®) is used at various concentrations for tissue dissociation, generally ranging from 0.6 to 2.4 U/ml for various time points depending on the desired effect. Concentrations higher than 2.4 U/ml are not recommended.
Activity Greater than or equal to 4.0 units per mg dry weight

Unit Definition: One unit releases Folin positive amino acids equivalent to 1 μmol of tyrosine per minute from casein at 37°C, pH 7.5.

Specificity / Sensitivity

Neutral Protease (Dispase®) is suggested to separate skin epidermis from dermis leaving intact epithelial sheets. This mild enzyme is used for non-specific cleavage of peptide bonds containing leucine and phenylalanine.

Source / Purification

Neutral Protease (Dispase®) is produced by the bacterium Bacillus polymyxa and chromatographically purified prior to lyophilization.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not for Use in Diagnostic Procedures.
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To Purchase # 44439
Cat. # Size Qty. Price
50 mg