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9769
14-3-3 Family Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

14-3-3 Family Antibody Sampler Kit #9769

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14-3-3 Family Antibody Sampler Kit: Image 1

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 β/α Antibody.

14-3-3 Family Antibody Sampler Kit: Image 2

Isoform specificity of 14-3-3γ (D15B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each)were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 γ (D15B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).

14-3-3 Family Antibody Sampler Kit: Image 3

Western blot analysis of extracts from various cell lines using 14-3-3 ζ/δ (D7H5) Rabbit mAb.

14-3-3 Family Antibody Sampler Kit: Image 4

Western blot analysis of extracts from various cell types using 14-3-3 ε Antibody.

14-3-3 Family Antibody Sampler Kit: Image 5

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody.

14-3-3 Family Antibody Sampler Kit: Image 6

Isoform specificity of 14-3-3 η (D23B7) Rabbit mAb. Recombinant, purified, GST-tagged 14-3-3 protein isofoms (2 µg each) were resolved by SDS-PAGE, transferred to nitrocellulose and blotted with either 14-3-3 η (D23B7) Rabbit mAb (upper) or GST (91G1) Rabbit mAb #2625 (lower).

14-3-3 Family Antibody Sampler Kit: Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

14-3-3 Family Antibody Sampler Kit: Image 8

Western blot analysis of extracts from various cell lines using 14-3-3 γ (D15B7) Rabbit mAb.

14-3-3 Family Antibody Sampler Kit: Image 9

Immunohistochemical analysis of paraffin-embedded human GIST, using 14-3-3 tau Antibody.

14-3-3 Family Antibody Sampler Kit: Image 10

Western blot analysis of whole cell extracts from various cell lines using 14-3-3 η (D23B7) Rabbit mAb.

14-3-3 Family Antibody Sampler Kit: Image 11

Immunohistochemical analysis of paraffin-embedded human lung carcinoma, showing cytoplasmic and nuclear localization, using 14-3-3 tau Antibody.

14-3-3 Family Antibody Sampler Kit: Image 12

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using 14-3-3 tau Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

14-3-3 Family Antibody Sampler Kit: Image 13

Western blot analysis of extracts from HeLa, NIH/3T3, and C6 cells using 14-3-3 τ Antibody.

To Purchase # 9769T
Product # Size Price
9769T
1 Kit  (6 x 20 µl) $ 445

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
14-3-3 β/α Antibody 9636 20 µl
  • WB
H M R Mk 28 Rabbit 
14-3-3 γ (D15B7) Rabbit mAb 5522 20 µl
  • WB
H M R Mk Pg 27 Rabbit IgG
14-3-3 ζ/δ (D7H5) Rabbit mAb 7413 20 µl
  • WB
H M R Mk Pg 28 Rabbit IgG
14-3-3 ε Antibody 9635 20 µl
  • WB
H M R Mk 28 Rabbit 
14-3-3 τ Antibody 9638 20 µl
  • WB
  • IHC
H M R Mk 28 Rabbit 
14-3-3 η (D23B7) Rabbit mAb 5521 20 µl
  • WB
H M R Mk B Pg 27 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The 14-3-3 Family Antibody Sampler Kit provides an economical means to investigate the expression of various 14-3-3 isoforms within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the 14-3-3 Family Antibody Sampler Kit detects endogenous levels of its respective target.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to the sequences of human 14-3-3 ß/α, 14-3-3 ε and 14-3-3 τ proteins. Antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg80 of human 14-3-3 ζ/δ protein, Leu37 of human 14-3-3 η protein and Ile79 of human 14-3-3 γ protein.

Background

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways (1,2). 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ, and η that have been identified in mammals. The initially described α and δ isoforms are confirmed to be phosphorylated forms of β and ζ, respectively (3). Through their amino-terminal α helical region, 14-3-3 proteins form homo- or heterodimers that interact with a wide variety of proteins: transcription factors, metabolic enzymes, cytoskeletal proteins, kinases, phosphatases, and other signaling molecules (3,4). The interaction of 14-3-3 proteins with their targets is primarily through a phospho-Ser/Thr motif. However, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation independent interactions has been observed (4). 14-3-3 binding masks specific sequences of the target protein, and therefore, modulates target protein localization, phosphorylation state, stability, and molecular interactions (1-4). 14-3-3 proteins may also induce target protein conformational changes that modify target protein function (4,5). Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in development and in acute response to extracellular signals and drugs, suggesting that 14-3-3 isoforms may perform different functions despite their sequence similarities (4). Several studies suggest that 14-3-3 isoforms are differentially regulated in cancer and neurological syndromes (2,3).

  1. Muslin, A.J. and Xing, H. (2000) Cell Signal 12, 703-9.
  2. Mackintosh, C. (2004) Biochem J 381, 329-42.
  3. Dougherty, M.K. and Morrison, D.K. (2004) J Cell Sci 117, 1875-84.
  4. Yaffe, M.B. (2002) FEBS Lett 513, 53-7.
  5. Bridges, D. and Moorhead, G.B. (2004) Sci STKE 2004, re10.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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