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26596
4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody
R
Recombinant

4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free) #26596

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  1. IHC
Immunohistochemistry Image 1: 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb performed on the Leica® BOND Rx.  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Raji cell pellet (left, positive) or A-172 cell pellet (right, negative) using 4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb. Data were generated using the standard formulation of this product.
To Purchase # 26596
Cat. # Size Qty. Price
26596SF
100 µg
$ 758

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 28
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • C&R-CUT&RUN
  • C&T-CUT&Tag
  • DB-Dot Blot
  • eCLIP-eCLIP
  • IF-Immunofluorescence
  • F-Flow Cytometry

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • GP-Guinea Pig
  • Rab-Rabbit
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #90204. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free.

For standard formulation of this product see product #90204

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity / Sensitivity

4-1BBL/CD137L/TNFSF9 (E8W3P) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total 4-1BBL/CD137L/TNFSF9 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human 4-1BBL/CD137L/TNFSF9 protein.

Background

TNFRSF9 is a member of the tumor necrosis factor receptor superfamily (1,2). It is also called 4-1BB or CD137 (1, 2). 4-1BB/CD137/TNFRSF9 is expressed in activated CD4+ and CD8+ T cells, natural killer cells and dendritic cells (2-5). The ligand 4-1BBL/CD137L/TNFSF9 on antigen presenting cells binds to 4-1BB/CD137/TNFRSF9 and costimulates the activation of T cells (5). The binding of agonistic antibodies to 4-1BB/CD137/TNFRSF9 also leads to costimulation for T cell activation (5). Studies have shown the effectiveness of targeting 4-1BB/CD137/TNFRSF9 by its agonistic antibodies in cancer immunotherapy (6).
  1. Sun, Y. et al. (2002) Nat Med 8, 1405-13.
  2. Wilcox, R.A. et al. (2002) J Clin Invest 109, 651-9.
  3. Lin, W. et al. (2008) Blood 112, 699-707.
  4. Wilcox, R.A. et al. (2002) J Immunol 168, 4262-7.
  5. Melero, I. et al. (1997) Nat Med 3, 682-5.
  6. Yonezawa, A. et al. (2015) Clin Cancer Res 21, 3113-20.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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