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Dec 31st Santa Cruz will discontinue a
large number of polyclonal products as
a result of the USDA settlement that was
made public May 19th.

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We recommend the following alternatives

W           H Mk
W IP     F   H Mk
W           H Mk Pg
W IP IHC IF F   H Mk
REACTIVITY SENSITIVITY MW (kDa) SOURCE
138 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Axl Antibody detects endogenous levels of Axl protein. The antibody does not cross-react with related proteins.


Species predicted to react based on 100% sequence homology: Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequences surrounding amino acid residue 740 of human Axl. Antibodies are purified by protein A and peptide affinity chromatography.

Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).


1.  Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-5.

2.  Demarchi, F. et al. (2001) J Biol Chem 276, 31738-44.

3.  Lee, W.P. et al. (2002) Oncogene 21, 329-36.

4.  Bellosta, P. et al. (1997) Oncogene 15, 2387-97.


Entrez-Gene Id 558
Swiss-Prot Acc. P30530


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

4977
Axl Antibody