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PhosphoSitePlus® Resource

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REACTIVITY SENSITIVITY MW (kDa) Isotype
66 Rabbit 

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb detects endogeneous levels of ERα protein only when phosphorylated at Ser167. The antibody cross reacts with a nonspecific band at around 77 kDa.


Species predicted to react based on 100% sequence homology: Monkey

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 of human estrogen receptor α protein.

Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains (1). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery (2). Phosphorylation at multiple sites provides an important mechanism to regulate ERα activity (3-5). Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regulating ERα activity. Ser118 may be the substrate of the transcription regulatory kinase CDK7 (5). Ser167 may be phosphorylated by p90RSK and Akt (4,6). According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients (4).


1.  Mangelsdorf, D.J. et al. (1995) Cell 83, 835-9.

2.  Glass, C.K. and Rosenfeld, M.G. (2000) Genes Dev 14, 121-41.

3.  Chen, D. et al. (1999) Mol Cell Biol 19, 1002-15.

4.  Campbell, R.A. et al. (2001) J Biol Chem 276, 9817-24.

5.  Chen, D. et al. (2000) Mol Cell 6, 127-37.

6.  Joel, P.B. et al. (1998) Mol Cell Biol 18, 1978-84.


Entrez-Gene Id 2099
Swiss-Prot Acc. P03372


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

5587
Phospho-Estrogen Receptor α (Ser167) (D1A3) Rabbit mAb