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REACTIVITY SENSITIVITY MW (kDa) SOURCE
43 Rabbit

Product Usage Information

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

p38δ MAPK Antibody detects endogenous levels of total p38δ MAPK protein. This antibody does not cross-react with other isoforms of p38 MAPK.


Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of p38δ MAPK. Antibodies are purified by protein A and peptide affinity chromatography.

p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).

SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).


1.  Rouse, J. et al. (1994) Cell 78, 1027-37.

2.  Han, J. et al. (1994) Science 265, 808-11.

3.  Lee, J.C. et al. (1994) Nature 372, 739-46.

4.  Freshney, N.W. et al. (1994) Cell 78, 1039-49.

5.  Raingeaud, J. et al. (1995) J Biol Chem 270, 7420-6.

6.  Zervos, A.S. et al. (1995) Proc Natl Acad Sci U S A 92, 10531-4.

7.  Zhao, M. et al. (1999) Mol Cell Biol 19, 21-30.

8.  Yang, S.H. et al. (1999) Mol Cell Biol 19, 4028-38.

9.  Cuenda, A. et al. (1995) FEBS Lett 364, 229-33.

10.  Kumar, S. et al. (1999) Biochem Biophys Res Commun 263, 825-31.


Entrez-Gene Id 5603
Swiss-Prot Acc. O15264


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

9214
p38δ MAPK Antibody