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29087
PhosphoPlus® α-Synuclein (Ser129) Antibody Duet
Primary Antibodies

PhosphoPlus® α-Synuclein (Ser129) Antibody Duet #29087

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Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green), in the presence of Phospho-α-Synuclein (Ser129) peptide (middle) or Non-phospho-α-Synuclein (Ser129) peptide (right). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of normal rat cerebellum, hippocampus and striatum using α-Synuclein (D37A6) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Confocal immunofluorescent analysis of untreated (left) or λ phosphatase-treated (right) mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper), or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Immunohistochemical analysis of paraffin-embedded mouse brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Confocal immunofluorescent analysis of mouse hippocampus using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (green). High-magnification showing punctate and sparse nuclear labeling (arrows). Specificity of nuclear staining is unclear (see Specificity/Sensitivity statement and supporting IF-F validation data). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from mouse and rat brain using α-Synuclein (D37A6) XP® Rabbit mAb.

Western blot analysis of extracts from mouse brain, untreated (-) or λ phosphatase-treated (+), using Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb (upper) or α-Synuclein (D37A6) XP® Rabbit mAb #4179 (lower).

To Purchase # 29087S
Product # Size Price
29087S
1 Kit $ 521

Product Includes Quantity Reactivity MW(kDa) Isotype
Phospho-α-Synuclein (Ser129) (D1R1R) Rabbit mAb 23706 100 µl H M R 18 Rabbit IgG
α-Synuclein (D37A6) XP® Rabbit mAb 4179 100 µl M R 18 Rabbit IgG

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

α-Synuclein is a protein of 140-amino acids expressed abundantly in the brain. α-Synuclein is also the main component of pathogenic Lewy bodies and Lewy neurites. Research studies have shown that mutations of the α-synuclein gene are linked to Parkinson's disease (1).

Various research studies have shown that phosphorylation of α-Synuclein at Ser129 is a highly toxic event that causes degeneration of dopaminergic neurons, which are associated with Parkinson's disease. This is proposed to occur through increased misfolding, aggregation, and accumulation of α-Synuclein phosphorylated at this site (2). GSK-3β is one of several kinases that has been reported to phosphorylate α-Synuclein at Ser129 (3).

  1. Goldberg, M.S. and Lansbury, P.T. (2000) Nat Cell Biol 2, E115-9.
  2. Oueslati, A. et al. (2010) Prog Brain Res 183, 115-45.
  3. Credle, J.J. et al. (2015) Cell Death Differ 22, 838-51.
  4. Yu, S. et al. (2007) Neuroscience 145, 539-55.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

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