Render Target: STATIC
Render Timestamp: 2024-10-09T10:34:36.246Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77

ACE2 (OTI1G4) Mouse mAb #74512

Filter:
  • WB
  • IHC

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 120-135
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:2000
    IHC Leica Bond 1:400 - 1:1600
    Immunohistochemistry (Paraffin) 1:200 - 1:800

    Storage

    Supplied in PBS (pH 7.3), 1% BSA, 0.02% sodium azide, and 50% glycerol. Stable for 12 months when stored at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ACE2 (OTI1G4) Mouse mAb recognizes endogenous levels of ACE2 protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to amino acids 18-237 of human ACE2 protein.

    Background

    ACE2 is a carboxypeptidase that catalyses the conversion of angiotensin I to angiotensin 1-9, or of angiotensin II to the vasodilator angiotensin 1-7 (1). ACE2 is a critical component in the renin-angiotensin system (RAS). ACE2 is predominantly expressed in vascular endothelial cells of the heart and kidney and Leydig and Sertoli cells of the testis (2,3). The unique expression pattern of ACE2 determines its essential role in the regulation of cardiovascular and kidney functions, as well as fertility. ACE2 protein is localized mainly in the extracellular space with its carboxy terminal end attached to the membrane via its transmembrane domain. Active ACE2 enzyme is secreted by cleavage at the amino terminus. Research studies have shown that ACE2 expression is elevated in human failing heart (4). ACE2 has also been identified as the receptor for SARS and SARS-CoV-2 coronaviruses (5-7).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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