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Render Timestamp:
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5/22/2026, 10:04:20 AM UTC
Commit: 7ed46ecc04b401f23a28df741b5078df405d23e4
XML generation date: 2025-11-13 17:57:42.829
Product last modified at: 2026-03-20T08:04:43.290Z
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PDP - Template Name: Polyclonal Antibody
PDP - Template ID: *******59c6464

Acetyl-Histone H2B (Lys12) Antibody #2575

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  • WB

Inquiry Info. # 2575

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    Product Specifications

    REACTIVITY H M R Mk
    SENSITIVITY
    MW (kDa) 14
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Acetyl-Histone H2B (Lys12) Antibody detects endogenous levels of histone H2B only when acetylated at lysine 12. This antibody does not cross-react with other acetylated histones.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys12 of human histone H2B. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).

    Alternate Names

    H2B; H2B clustered histone 3; H2B histone family, member F; H2B-clustered histone 3; H2B.1; H2B/f; H2B1B; H2BC3; H2BFF; HIST1H2BB; histone 1, H2bb; histone cluster 1 H2B family member b; histone cluster 1, H2bb; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; MGC119804

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