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Ack1 Antibody #3131

Inquiry Info. # 3131

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    Product Specifications

    REACTIVITY
    SENSITIVITY Endogenous
    MW (kDa) 100, 135
    SOURCE Rabbit

    Product Information

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Specificity / Sensitivity

    Ack1 Antibody detects endogenous levels of total Ack1 protein. This antibody may cross-react with Ack2 but not other related proteins.

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Pro90 of human Ack1. Antibodies are purified by peptide affinity chromatography.

    Background

    Ack1 and Ack2 (activated cdc42-associated kinase 1 and 2) are non-receptor tyrosine kinases that consist of a tyrosine kinase core, an SH3 domain, a cdc42/Rac-binding (CRIB) domain, a Ralt homology region and a proline-rich region (1,2). Ack1 and 2 are the only two tyrosine kinases known to interact with cdc42. Both Acks are activated by growth factors including EGF and PDGF, as well as by activated integrins through cell adhesion, and may serve to link receptor tyrosine kinase or G protein-coupled receptor signaling with cdc42. Acks may regulate cell growth, morphology and motility (3,4). Recent findings indicate that Ack1 may play a role in prostate tumorigenesis, making it a potential drug target for this type of cancer (5).

    Alternate Names

    ACK; ACK-1; ACK1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK2; tyrosine kinase non receptor 2; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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