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|H M Mk||Endogenous||57||Rabbit|
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised November 2013
Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available. It should be noted that for the best possible results a fresh blot is always recommended. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalently purified water.
posted June 2005
revised October 2016
Protocol Id: 10
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
ACVR1 Antibody detects endogenous levels of total ACVR1 protein. The antibody also cross-reacts with 35 kDa and 95 kDa bands of unknown origin.
Human, Mouse, Monkey
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ACVR1 protein. Antibodies were purified by protein A and peptide affinity chromatography.
The activin A receptor type 1 (ACVR1) is a membrane associated serine/threonine kinase receptor that binds activin A and related TGF-β superfamily growth factors, including bone morphogenetic protein 2 (BMP2) and BMP4, 6 and 7 (1-3). Functional activin receptors are composed of a pair of type I and type II activin receptor proteins. Following ligand binding, ACVR1 is phosphorylated by its type II binding partner, which allows recruitment and activation of receptor regulated SMAD (R-Smad) proteins (4). Phosphorylation of R-Smad proteins results in their translocation to the nucleus where R-Smads stimulate transcription of target genes (5). Mutations in the corresponding ACVR1 gene are associated with the genetic developmental disorders hereditary hemorrhagic telangiectasia and fibrodysplasia ossificans progressive (6,7).
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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