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  3. ALCAM (F9J1I) Rabbit Monoclonal Antibody
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Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ALCAM (F9J1I) Rabbit Monoclonal Antibody #27937

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  • WB
  • F

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 100
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Flow Cytometry (Fixed/Permeabilized) 1:100 - 1:400
    Flow Cytometry (Live) 1:100 - 1:400

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALCAM (F9J1I) Rabbit Monoclonal Antibody recognizes endogenous levels of total ALCAM protein.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human ALCAM protein.

    Background

    Activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 or MEMCAM, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed in various cell types, including leukocytes, fibroblasts, epithelial cells, and neurons. ALCAM functions primarily as a cell adhesion molecule, mediating both homotypic (ALCAM-ALCAM) and heterotypic interactions with other cell surface receptors, most notably CD6 (ALCAM-CD6). These interactions, and others, play critical roles in various physiological and pathological processes, including hematopoiesis, neuronal development and function, T cell activation and proliferation, immune response, angiogenesis, and tumor progression and metastasis (1-5). Aberrant ALCAM expression is frequently observed in various human tumors, including melanoma, prostate cancer, breast cancer, colorectal carcinoma, bladder cancer, and pancreatic adenocarcinoma. High ALCAM expression correlates with poor prognosis and increased metastatic potential in many of these tumor types (5). ALCAM can mediate tumor-endothelial cell interactions, facilitating tumor cell dissemination and extravasation to distant sites such as the brain, bone, and liver. It has been implicated in promoting tumor cell invasion, migration, and survival in the tumor microenvironment and may contribute to chemotherapy drug resistance in some cancers (5).

    Alternate Names

    activated leucocyte cell adhesion molecule; Activated leukocyte cell adhesion molecule; ALCAM; CD166; CD166 antigen; FLJ38514; MEMD; MGC71733

    Database Links

    UniProt ID: Q13740


    Entrez-Gene Id: 214


    Limited Uses

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    For Research Use Only. Not for Use in Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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