ALDH1L1 (E7I2Q) Rabbit mAb #85828
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 98 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
ALDH1L1 (E7I2Q) Rabbit mAb recognizes endogenous levels of total ALDH1L1 protein. This antibody detects a non-specific band of unknown origin in rodent tissue extracts at 47 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val431 of human ALDH1L1 protein.
Background
Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH), is a member of the aldehyde dehydrogenase super family. It is a key enzyme in folate metabolism, playing an important role in regulation of cellular metabolism including proliferation (1,2). Downregulation of ALDH1L1 has been seen in tumors, leading to decreased suppression of cancer cell proliferation (2,3).
ALDH1L1 has also been shown to be a useful astrocyte marker throughout the gray and white matter of the brain, labeling both the cell body and processes of astrocytes. ALDH1L1 does not label oligodendrocytes or neurons (4). It has been shown that expression of ALDH1L1 in astrocytes is stably expressed through normal tissue and during astrogliosis (5).
Limited Uses
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