Render Target: STATIC
Render Timestamp: 2024-10-09T11:11:43.838Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ALDH1L1 (E7I2Q) Rabbit mAb #85828

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 98
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:50
    Immunofluorescence (Frozen) 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ALDH1L1 (E7I2Q) Rabbit mAb recognizes endogenous levels of total ALDH1L1 protein. This antibody detects a non-specific band of unknown origin in rodent tissue extracts at 47 kDa.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val431 of human ALDH1L1 protein.

    Background

    Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), also known as 10-formyltetrahydrofolate dehydrogenase (FDH), is a member of the aldehyde dehydrogenase super family. It is a key enzyme in folate metabolism, playing an important role in regulation of cellular metabolism including proliferation (1,2). Downregulation of ALDH1L1 has been seen in tumors, leading to decreased suppression of cancer cell proliferation (2,3).

    ALDH1L1 has also been shown to be a useful astrocyte marker throughout the gray and white matter of the brain, labeling both the cell body and processes of astrocytes. ALDH1L1 does not label oligodendrocytes or neurons (4). It has been shown that expression of ALDH1L1 in astrocytes is stably expressed through normal tissue and during astrogliosis (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    Alexa Fluor is a registered trademark of Life Technologies Corporation.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.