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8652
AMPA Receptor (GluA) Antibody Sampler Kit

AMPA Receptor (GluA) Antibody Sampler Kit #8652

Western Blotting Image 1

Western blot analysis of extracts from mouse brain, rat brain, and rat prefrontal cortex tissues using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.

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Western Blotting Image 2

Western blot analysis of extracts from mouse and rat brains using AMPA Receptor 2 (GluA2) (D39F2) Rabbit mAb.

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Western Blotting Image 3

Western blot analysis of extracts from mouse and rat brains using AMPA Receptor 3 (GluA 3) (D47E3) Rabbit mAb.

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Western Blotting Image 4

Western blot analysis of extracts from mouse brain, NIH/3T3 cells, rat brain, and C6 cells using AMPA Receptor 4 (GluA 4) (D41A11) XP® Rabbit mAb.

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Western Blotting Image 5

Western blot analysis of extracts from mouse brain and rat brain using Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb. The phospho-specificity of the antibody was verified by blocking with a phospho or nonphosphopeptide.

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Western Blotting Image 6

Western blot analysis of extracts from rat brain, either 15 min ischemia followed by 4 h reperfusion or 15 min ischemia only, using Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody (upper) or AMPA Receptor (GluA2/3/4) Antibody #2460 (lower).

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Western Blotting Image 7

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

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IP Image 8

Immunoprecipitation of AMPA Receptor 1 (GluA1) from mouse brain extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb.

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IF-F Image 9

Confocal immunofluorescent analysis of rat retina using AMPA Receptor (GluA 4) (D41A11) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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IF-F Image 10

Confocal immunofluorescent analysis of mouse hippocampus using AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb 13185 20 µl
  • WB
  • IP
  • IF
H M R 100 Rabbit IgG
AMPA Receptor 2 (GluA2) (D39F2) Rabbit mAb 5306 20 µl
  • WB
H M R 100 Rabbit IgG
AMPA Receptor 3 (GluA 3) (D47E3) Rabbit mAb 4676 20 µl
  • WB
  • IP
H M R 100 Rabbit IgG
AMPA Receptor 4 (GluA 4) (D41A11) XP® Rabbit mAb 8070 20 µl
  • WB
  • IP
  • IF
H M R 100 Rabbit IgG
Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb 8084 20 µl
  • WB
  • IP
H M R 100 Rabbit IgG
Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody 3921 20 µl
  • WB
R 100 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

The AMPA Receptor (GluA) Antibody Sampler Kit provides an economical means of evaluating the four subunits of AMPARs. The kit contains enough primary and secondary antibodies to perform two western blot experiments with each antibody.

Phospho-AMPA Receptor 1 (GluA1) (Ser845) (D10G5) Rabbit mAb recognizes endogenous levels of AMPA Receptor (GluR 1) protein only when phosphorylated at Ser845. While the literature refers to this residue as Ser845, it is Ser863 in the UniProt sequence P42261. Phospho-AMPA Receptor 2 (GluA2) (Tyr869/Tyr873/Tyr876) Antibody detects endogenous levels of GluR 2 protein only when phosphorylated at Tyr869, Tyr873, or Tyr876. It may also detect GluA 3 when phosphorylated at the conserved Tyr880, Tyr884, or Tyr887. These residues are not conserved in GluR 1 or GluR 4. AMPA Receptor (GluR 2) (D39F2) Rabbit mAb detects endogenous levels of total GluR 2 protein. The antibody is not predicted to recognize other AMPA receptor subunits (e.g. GluA1, GluA3, or GluA4) based on sequence homology of the antigen. AMPA Receptor 3 (GluA3) (D47E3) Rabbit mAb detects endogenous levels of total GluR 3 protein. The antibody is not predicted to detect other AMPA receptor subunits (e.g. GluA1, GluA2, or GluA4) based on sequence homology of the antigen. AMPA Receptor 4 (GluA4) (D41A11) XP® Rabbit mAb detects endogenous levels of total GluR 4 protein. AMPA Receptor 1 (GluA1) (D4N9V) Rabbit mAb recognizes endogenous levels of total AMPA Receptor (GluA1) protein.

Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr869, Tyr873, and Tyr876 of human GluA2 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human GluA2 protein, surrounding Pro590 of human GluA3 protein, surrounding Gln890 of human GluA4 protein, surrounding Ser845 of human AMPA Receptor (GluA1) protein or surrounding Ala275 of human AMPA Receptor (GluA1) protein.

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).

  1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
  2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
Entrez-Gene Id
2890
Swiss-Prot Acc.
P42261
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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To Purchase # 8652T

Product Number Size Price
8652T 1 Kit (6 x 20 µl) $409.00.0
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