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REACTIVITY SENSITIVITY MW (kDa) SOURCE
H M R Endogenous 100 Rabbit

Product Usage Information

Application Dilutions
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

AMPA Receptor 4 (GluR 4) (Arg860) Antibody detects endogenous levels of total GluR 4 protein.


Species Reactivity: Human, Mouse, Rat

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg860 of human GluR 4. Antibodies are purified by protein A and peptide affinity chromatography.

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).


GluR 4 containing AMPA receptors are found in synapses and GluR 4 delivery to synapses and cell surface expression is mediated through phosphorylation of Ser842 by PKA or PKC (3).


1.  Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.

2.  Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.

3.  Gomes, A.R. et al. (2007) Traffic 8, 259-269.


Entrez-Gene Id 2893
Swiss-Prot Acc. P48058


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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AMPA Receptor 4 (GluA 4) (Arg860) Antibody