Render Target: STATIC
Render Timestamp: 2024-12-06T10:41:34.830Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-30 01:55:25.031
Product last modified at: 2024-10-17T18:45:13.482Z
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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

Angiomotin (D2O4H) Rabbit mAb #43130

Filter:
  • WB
  • IP
  • IF

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 140, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunofluorescence (Immunocytochemistry) 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Angiomotin (D2O4H) Rabbit mAb recognizes endogenous levels of total Angiomotin protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with the AMOT family members AMOTL1 or AMOTL2.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near Pro1049 of human Angiomotin protein.

    Background

    Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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