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Angiomotin (F7W8U) Rabbit Monoclonal Antibody #30020

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  • IP
  • IHC
  • IF
  • F

    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 140, 80
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:250 - 1:1000
    Immunofluorescence (Frozen) 1:800 - 1:1600
    Immunofluorescence (Immunocytochemistry) 1:200 - 1:400
    Flow Cytometry (Fixed/Permeabilized) 1:400 - 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Angiomotin (F7W8U) Rabbit Monoclonal Antibody recognizes endogenous levels of total angiomotin protein. Based on the sequence of the immunogenic peptide, this antibody is not expected to cross-react with the AMOT family members AMOTL1 or AMOTL2.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1075 of human angiomotin protein.

    Background

    Angiomotin (AMOT) is a widely expressed cell junction protein initially identified through its ability to bind angiostatin. Alternative splicing results in two isoforms of angiomotin, the full-length p130 and the amino-terminally truncated p80. These isoforms, along with angiomotin-like 1 (AMOTL1) and angiomotin-like 2 (AMOTL2), comprise the Motin protein family. Angiomotin is important in endothelial cell polarity, migration, and blood vessel formation during development, as well as in signaling through small GTPases and the Hippo/YAP pathway (reviewed in 1). Research studies have shown that angiomotin expression regulates migration and proliferation of breast cancer cells (2,3).

    Alternate Names

    AMOT; Angiomotin; angiomotin p130 isoform; angiomotin p80 isoform; KIAA1071

    For Research Use Only. Not for Use in Diagnostic Procedures.
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