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APEX2 (E5A2Z) Rabbit Monoclonal Antibody #74728

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  • WB
  • IP

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 62
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    APEX2 (E5A2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total APEX2 protein. This antibody cross-reacts with an aproximately 100 kDa band of unknown origin by western blot.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu393 of human APEX2 protein.

    Background

    DNA-(apurinic or apyrimidinic site) endonuclease 2 (APEX2/APE2) is an endodeoxyribonuclease that functions in the DNA base excision repair (BER) pathway to repair DNA lesions induced by oxidative and alkylating agents. APEX2 acts in ATR-dependent DNA damage checkpoint activation in response to oxidative stress (1). The APEX2 gene is synthetically lethal with BRCA1 and BRCA2 genes, which are required for homologous recombination (HR) repair of double-strand DNA breaks. This is due to the ability of APEX2 to reverse single-strand DNA breaks with 3’ end blocks. Because BRCA-deficient cancer cells are sensitive to 3’ blocks, APEX2 is a potential therapeutic target for HR-deficient cancers (2,3).

    Alternate Names

    AP endonuclease 2; AP endonuclease XTH2; APE2; APEX nuclease (apurinic/apyrimidinic endonuclease) 2; APEX nuclease 2; APEX nuclease-like 2; APEX2; APEXL2; Apurinic-apyrimidinic endonuclease 2; apurinic/apyrimidinic endodeoxyribonuclease 2; apurinic/apyrimidinic endonuclease-like 2; DNA-(apurinic or apyrimidinic site) endonuclease 2; DNA-(apurinic or apyrimidinic site) lyase 2; XTH2; ZGRF2; zinc finger, GRF-type containing 2

    For Research Use Only. Not for Use in Diagnostic Procedures.
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