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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 9664 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H M R Mk 17, 19 Rabbit IgG
Caspase-3 (8G10) Rabbit mAb 9665 20 µl
Western Blotting Immunoprecipitation
H M R Mk 17, 19, 35 Rabbit IgG
PARP Antibody 9542 20 µl
Western Blotting
H M R Mk 89, 116 Rabbit 
Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb 5625 20 µl
Western Blotting Immunoprecipitation Immunohistochemistry Immunofluorescence Flow Cytometry
H Mk 89 Rabbit IgG
Caspase-9 (C9) Mouse mAb 9508 20 µl
Western Blotting
H M R Hm Mk 47 /37/35 (H). 51 /39/37 (R,M). Mouse IgG1
Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb 7237 20 µl
Western Blotting Immunoprecipitation
H 37 Rabbit IgG
Caspase-7 (D2Q3L) Rabbit mAb 12827 20 µl
Western Blotting
H M R 20, 35 Rabbit IgG
Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 8438 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R 18 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
Western Blotting
Horse 

Product Description

The Apoptosis Antibody Sampler Kit provides an economical means to evaluate the levels of inactive and active caspases. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each antibody.


Specificity / Sensitivity

Each antibody in the Apoptosis Antibody Sampler Kit detects endogenous levels of its respective target. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb, Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb, Cleaved Caspase-9 (Asp330) (D2D4) Rabbit mAb, and Cleaved PARP (Asp214) (D64E10) XP® Rabbit mAb detect only the large cleaved fragments of their respective targets. Caspase-3 (8G10) Rabbit mAb, Caspase-7 (D2Q3L) Rabbit mAb, Caspase-9 (C9) Mouse mAb, and PARP Antibody detect both the full length and the large cleaved fragments of their respective targets.


Source / Purification

Monoclonal and polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Pro158 of human caspase-7 protein, residues surrounding the proteolytic cleavage sites of human caspase-3, caspase-9 and PARP proteins, or with recombinant human caspase-9 protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase (1). Proapoptotic stimuli include the FasL, TNF-α, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10 (2), DNA damage leads to the activation of caspase-9 and ER stress leads to the calcium-mediated activation of caspase-12 (3). The inhibitor of apoptosis protein (IAP) family includes XIAP and survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6).


1.  Baker, S.J. and Reddy, E.P. (1998) Oncogene 17, 3261-3270.

2.  Budihardjo, I. et al. (1999) Annu. Rev. Cell Dev. Biol. 15, 269-290.

3.  Nakagawa, T. et al. (2000) Nature 403, 98-103.

4.  Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.

5.  Li, F. et al. (1998) Nature 396, 580-4.

6.  Du, C. et al. (2000) Cell 102, 33-42.



For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 5,675,063.

9915
Apoptosis Antibody Sampler Kit