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  3. Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit

Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit #9660

Inquiry Info. # 9660

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    Product Information

    Kit Usage Information

    Protocols

    Product Description

    Apoptosis Marker: Cleaved Caspase-3 (Asp175) Western Detection Kit offers an efficient way of detecting caspase-3 processing and activation by Western blotting. The kit contains enough primary and secondary antibodies to perform 10 Western mini blots, as well as a set of pre-stained and biotinylated markers, cell lysates and LumiGLO® reagent.

    Specificity / Sensitivity

    Cleaved Caspase-3 (Asp175) Antibody detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to (Asp175). This antibody does not recognize full length caspase-3 or other cleaved caspases. Caspase-3 Antibody detects endogenous levels of full length caspase-3 (35 kDa) and the large fragment of caspase-3 resulting from cleavage (17 kDa).

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding the cleavage site and the amino terminus of the large fragment of human caspase-3. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

    Alternate Names

    Apopain; casp367; casp39; caspase 3; caspase-3; caspase3; CPP 32; CPP-32; CPP32; SCA-1; Yama Protein

    Database Links

    UniProt ID: P42574


    Entrez-Gene Id: 836


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    For Research Use Only. Not for Use in Diagnostic Procedures.
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