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ARID5A (F7T9F) Rabbit Monoclonal Antibody

ARID5A (F7T9F) Rabbit Monoclonal Antibody #35393

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Product Specifications

REACTIVITY	H
SENSITIVITY	Endogenous
MW (kDa)	75
Source/Isotype	Rabbit IgG

Application Key:

WB-Western Blotting
IP-Immunoprecipitation

Species Cross-Reactivity Key:

H-Human

Product Information

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Protocol

Specificity / Sensitivity

ARID5A (F7T9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ARID5A protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu154 of human ARID5A protein.

Background

AT-rich interactive domain-containing protein 5A (ARID5A) is a member of the ARID family, which includes 15 distinct human proteins. ARID is a nucleic acid binding domain that has a helix-turn-helix motif and is conserved in all eukaryotes (1,2). ARID5A expression is seen in many immune cells, including macrophages, CD4⁺ T cell subsets TH1 and TH17, as well as stromal cells and keratinocytes (2). During inflammation, ARID5A is translocated from the nucleus to the cytoplasm and stabilizes a variety of mRNA transcripts, including IL-6, Stat3, OX40, T-bet, and IL-17, contributing to the inflammatory response and a variety of inflammatory diseases. TLR4-activated NF-kB and MAPK pathways are involved in regulating ARID5A expression (3). ARID5A plays an important role in resistance to chimeric antigen receptor (CAR)-T cell therapy by stabilizing IDO1 mRNA and the upregulation of IDO1 expression. Elevated IDO1 expression levels facilitate the conversion of tryptophan to kynurenine, which contributes to CAR-T cell exhaustion (4).

Alternate Names

ARI5A; ARID domain-containing protein 5A; ARID5A; AT rich interactive domain 5A; AT rich interactive domain 5A (MRF1-like); AT-rich interaction domain 5A; AT-rich interactive domain-containing protein 5A; Modulator recognition factor 1; modulator recognition factor I; MRF-1; MRF1; RFVG5814; RP11-363D14

Database Links

UniProt ID: Q03989

Entrez-Gene Id: 10865

Limited Uses

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