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Aromatase Antibody #8799

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  • IP

Inquiry Info. # 8799

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 51
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Aromatase Antibody recognizes endogenous levels of total aromatase protein. This antibody also cross-reacts with a 32 kDa protein of unknown origin in some cells.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human aromatase protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Aromatase is a member of the cytochrome P450 superfamily of enzymes, which are monooxygenases that catalyze reactions involved in drug metabolism and cholesterol and steroid synthesis (1,2). Aromatase is responsible for the conversion of testosterone into 17-β estradiol (2). Aromatase is mainly expressed in the brain (3), ovaries (4), and placenta (5). Aromatase plays an important role in development of the central nervous system during ontogenesis (6,7), gonadal development, and sex differentiation (8,9). Research studies have suggested that inhibition of aromatase may be an effective therapeutic strategy for postmenopausal breast cancers that are estrogen receptor positive (6,10). Mutations in the corresponding aromatase gene are associated with cases of aromatase excess syndrome (AEXS) and aromatase deficiency (AROD) disorders (11-14).

    Alternate Names

    ARO; ARO1; Aromatase; CP19A; CPV1; CYAR; CYP19; CYP19A1; CYPXIX; Cytochrome P-450AROM; Cytochrome P450 19A1; cytochrome P450 family 19 subfamily A member 1; cytochrome P450, family 19, subfamily A, polypeptide 1; cytochrome P450, subfamily XIX (aromatization of androgens); Estrogen synthase; estrogen synthetase; flavoprotein-linked monooxygenase; MGC104309; microsomal monooxygenase; P-450AROM

    For Research Use Only. Not for Use in Diagnostic Procedures.
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