Western blot analysis of extracts from THP-1 and HL-60 cells, untreated (-) or treated overnight with TPA #9905 (+), using ASM Antibody.
Western blot analysis of extracts from COS-7 cells, untransfected (-) or transfected with a human ASM construct (+), using ASM Antibody.
|MW (kDa)||57, 70|
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
ASM Antibody detects endogenous levels of total human ASM protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxyl terminus of human ASM. Antibody was purified by protein A and peptide affinity chromatography.
Sphingomyelinases (SMases) catalyze the hydrolysis of sphingomyelin to produce ceramide and phosphocholine (1). Ceramide is an important bioactive lipid triggering signal transduction involved in cell proliferation, apoptosis and differentiation (1,2). A number of SMases have been described and categorized based on their optimum pH activity, cation dependence, tissue distribution, and subcellular localization (1). These include a lysosomal acid SMase, a Zn++-dependent secreted acid SMase, a membrane-bound Mg++-dependent neutral SMase, a Mg++-independent neutral SMase, and an alkaline SMase.
Acid sphingomyelinase (ASM or SMPD1) is a lysosomal enzyme responsible for the hydrolysis of sphingomyelin to ceramide and phosphocholine. The ASM gene encodes three proteins, ASM-1, ASM-2, and ASM-3, of which ASM-1 is the only catalytically active enzyme (3,4). ASM-1 can exist as a 70 kDa form as well as a 57 kDa proteoytic product (5). Expression of ASM is induced during monocytic cell differentiation (6). Defects in the ASM gene are associated with type A and type B Niemann-Pick disease (7).
Explore pathways + proteins related to this product.
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