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Astrin/SPAG5 Antibody #60940

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 135, 150
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Astrin/SPAG5 Antibody recognizes endogenous levels of total Astrin/SPAG5 protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val185 of human Astrin/SPAG5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Astrin/SPAG5 was identified as a microtubule-associated protein in a mitotic extract (1). It is essential for cells to assemble biploar spindle structures and progress through mitosis (1, 2). Astrin/SPAG5 was also identified to be a component associated with outer dense fibers in the sperm tail (3). In addition, this protein negatively regulates mTORC1 activity during the cell stress response (4). Under stress conditions, Astrin/SPAG5 interacts with the mTORC1 component raptor and recruits raptor to stress granules, thereby suppressing mTORC1 formation (4). The inhibition of mTORC1 prevents its hyperactivation and thus keeps cells from undergoing apoptosis during stresses (4). Furthermore, Astrin/SPAG5 has been implicated to be a prognostic marker in breast cancer (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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