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31068
PhosphoPlus® ATM (Ser1981) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® ATM (Ser1981) Antibody Duet #31068

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other Image 1 - PhosphoPlus® ATM (Ser1981) Antibody Duet

Western blot analysis of extracts of HeLa, NCCIT and PYS2 cells using ATM (D2E2) Rabbit mAb.

other Image 2 - PhosphoPlus® ATM (Ser1981) Antibody Duet

Flow cytometric analysis of HeLa cells, untreated (left) or treated with camptothecin (1 μM, 2 hr; right), using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb and Propidium Iodide (PI)/RNase Staining Solution #4087 to measure DNA content. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

other Image 3 - PhosphoPlus® ATM (Ser1981) Antibody Duet

Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with neocarzinostatin (NCS 10 μM, 1 hr; +), using Phospho-ATM (Ser1981) (D25E5) Rabbit mAb (upper) and ATM (D2E2) Rabbit mAb #2873 (lower).

To Purchase # 31068S
Product # Size Price
31068S
1 Kit $ 514

Product Includes Quantity Reactivity MW(kDa) Isotype
ATM (D2E2) Rabbit mAb 2873 100 µl H M 350 Rabbit IgG
Phospho-ATM (Ser1981) (D25E5) Rabbit mAb 13050 100 µl H 350 Rabbit IgG

Kit Usage Information

Protocols

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair (1). Activation of ATM by autophosphorylation on Ser1981 occurs in response to exposed DNA double stranded breaks. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair. Known substrates include p53, Chk2, Chk1, CtIP, 4E-BP1, BRCA1, RPA3, H2A.X, SMC1, FANCD2, Rad17, Artemis, Nbs1, and the I-2 regulatory subunit of PP1 (1,2). Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage (3,4).

  1. Lee, J.H. and Paull, T.T. (2007) Oncogene 26, 7741-8.
  2. Tang, X. et al. (2008) Mol Cell Biol 28, 2559-66.
  3. Shiloh, Y. (1997) Annu Rev Genet 31, 635-62.
  4. Petrini, J.H. (2000) Curr Opin Cell Biol 12, 293-6.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.