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For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 263
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu209 of human ATP5A1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
ATP synthase alpha-subunit (ATP5A1) is a component of the mitochondrial complex V, which functions to generate ATP from ADP. Generation of ATP is catalyzed by ATP synthase. ATP synthase is composed of a soluble catalytic core, F1, and a membrane-spanning proton channel, F0. ATP5A1 is one of five different subunits that comprise the catalytic F1 core. Interestingly, ATP5A1 is genetically linked to neurodevelopmental diseases (1). Moreover, the genetically linked mutant forms of C9ORF72, the most common genetic causes of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), bind to ATP5A1 and alter its protein expression, suggesting that mitochondrial dysfunction may be a major driver of disease in C9ORF72-linked ALS/FTD (2).
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