Render Target: STATIC
Render Timestamp: 2024-10-04T10:38:49.593Z
Commit: f04ddd7fea9fb3592f59f61482fcb94610d25cbe
1% for the planet logo
PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

ATRX (E5X7O) Rabbit mAb #10321

Filter:
  • WB
  • IP
  • IHC

    Supporting Data

    REACTIVITY H M R Mk
    SENSITIVITY Endogenous
    MW (kDa) 280
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    • IHC-Immunohistochemistry 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100
    Immunohistochemistry (Paraffin) 1:150 - 1:600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    ATRX (E5X7O) Rabbit mAb recognizes endogenous levels of total ATRX protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human ATRX protein.

    Background

    α-thalassemia/mental retardation X-linked (ATRX) is a transcriptional regulator and helicase that belongs to the SNF2 family of chromatin remodeling proteins (1,2). Together with its binding partner death-associated protein 6 (Daxx), ATRX acts as histone chaperone to deposit histone variant H3.3 at repetitive DNA sequences such as telomeric, pericentric, and ribosomal gene repeats (3-6). ATRX is involved in many nuclear functions that ensure proper sister chromatid cohesion during mitosis and chromosome alignment during meiosis (7,8). The ATRX transcriptional regulator also plays a role in the maintenance of telomere integrity and the regulation of gene expression during mammalian development by influencing DNA methylation patterns at high DNA repeat sequences (9,10). Mutations in the corresponding ATRX gene results in ATR-X syndrome, an X-linked disorder characterized by intellectual disabilities, craniofacial abnormalities, and mild α-thalassemia (11,12). Research studies indicate that the loss of ATRX protein occurs in numerous cancers, including pancreatic neuroendocrine tumors (PanNETs) and pediatric glioblastoma, where telomere maintenance occurs independently of telomerase (13-16).
    1. Clynes, D. et al. (2013) Trends Biochem Sci 38, 461-6.
    2. Picketts, D.J. et al. (1996) Hum Mol Genet 5, 1899-907.
    3. Drané, P. et al. (2010) Genes Dev 24, 1253-65.
    4. Elsässer, S.J. et al. (2012) Nature 491, 560-5.
    5. Lewis, P.W. et al. (2010) Proc Natl Acad Sci U S A 107, 14075-80.
    6. Goldberg, A.D. et al. (2010) Cell 140, 678-91.
    7. Ritchie, K. et al. (2008) J Cell Biol 180, 315-24.
    8. De La Fuente, R. et al. (2004) Dev Biol 272, 1-14.
    9. Wong, L.H. et al. (2010) Genome Res 20, 351-60.
    10. Gibbons, R.J. et al. (2000) Nat Genet 24, 368-71.
    11. Gibbons, R.J. et al. (1995) Cell 80, 837-45.
    12. Gibbons, R.J. et al. (1995) Hum Mol Genet 4 Spec No, 1705-9.
    13. Heaphy, C.M. et al. (2011) Science 333, 425.
    14. Lovejoy, C.A. et al. (2012) PLoS Genet 8, e1002772.
    15. Schwartzentruber, J. et al. (2012) Nature 482, 226-31.
    16. Jiao, Y. et al. (2011) Science 331, 1199-203.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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