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Aurora A (1F8) Mouse Monoclonal Antibody #12100

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    Product Specifications

    REACTIVITY H Mk
    SENSITIVITY Endogenous
    MW (kDa) 48
    Source/Isotype Mouse IgG1
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    • F-Flow Cytometry 
    Species Cross-Reactivity Key:
    • H-Human 
    • Mk-Monkey 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunofluorescence (Immunocytochemistry) 1:800
    Flow Cytometry (Fixed/Permeabilized) 1:1600

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Aurora A (1F8) Mouse Monoclonal Antibody recognizes endogenous levels of total Aurora A protein.

    Species Reactivity:

    Human, Monkey

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human Aurora A protein.

    Background

    Aurora A (AIK) is a cell cycle-regulated Ser/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment/segregation, and altered survivin localization (6).

    Alternate Names

    AIK; AIRK1; ARK-1; ARK1; AURA; AURKA; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; hARK1; IAK1; IPL1-related kinase; MGC34538; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine/threonine kinase 6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; STK15; STK6; STK7

    For Research Use Only. Not for Use in Diagnostic Procedures.
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