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To Purchase # 12738T

12738T 1 Kit (6 x 20 µl) $409.00


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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-CENP-A (Ser7) Antibody 2187 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H 17 Rabbit 
Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb 3377 20 µl
Western Blotting Immunofluorescence Flow Cytometry
H M R Mk Z 17 Rabbit IgG
Phospho-Histone H3 (Ser28) Antibody 9713 20 µl
Western Blotting Immunoprecipitation Immunofluorescence Flow Cytometry
H M Hm Dm 17 Rabbit 
Phospho-p53 (Ser315) Antibody 2528 20 µl
Western Blotting
H 53 Rabbit 
Phospho-PLK1 (Thr210) (D5H7) Rabbit mAb 9062 20 µl
Western Blotting Immunoprecipitation
H 62 Rabbit IgG
Phospho-TACC3 (Ser558) (D8H10) XP® Rabbit mAb 8842 20 µl
Western Blotting Immunofluorescence
H 140 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Aurora A/B Substrate Antibody Sampler Kit provides an economical means to investigate the G2/M phase of the cell cycle. The kit contains enough primary antibody to perform two western blots per primary antibody.

Specificity / Sensitivity

Each antibody in the Aurora Antibody Sampler Kit detects endogenous levels of its respective modification-specific target protein and does not cross-react with other family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide and are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with recombinant human proteins or synthetic peptides.

Aurora kinases belong to a highly conserved family of mitotic serine/threonine kinases with three members identified among mammals: Aurora A, B, and C (1,2). Studies on the temporal expression pattern and subcellular localization of Aurora kinases in mitotic cells suggest an association with mitotic structure. Aurora kinase functional influences span from G2 phase to cytokinesis and may be involved in key cell cycle events such as centrosome duplication, chromosome bi-orientation and segregation, cleavage furrow positioning, and ingression (3). Aurora A is detected at the centrosomes, along mitotic spindle microtubules, and in the cytoplasm of mitotically proliferating cells. Aurora A protein levels are low during G1 and S phases and peak during the G2/M phase of the cell cycle. Phosphorylation of Aurora A at Thr288 in its catalytic domain increases kinase activity. Aurora A is involved in centrosome separation, maturation, and spindle assembly and stability. Expression of Aurora B protein also peaks during the G2/M phase of the cell cycle; Aurora B kinase activity peaks at the transition from metaphase to the end of mitosis. Aurora B associates with chromosomes during prophase prior to relocalizing to the spindle at anaphase. Aurora B regulates chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. Expression of both Aurora A and Aurora B during the G2/M phase transition is tightly coordinated with histone H3 phosphorylation (4,5); research investigators have observed overexpression of these kinases in a variety of human cancers (2,4). Aurora C localizes to the centrosome from anaphase to cytokinesis and both mRNA and protein levels peak during G2/M phase. Although typical Aurora C expression is limited to the testis, research studies report overexpression of Aurora C is detected in various cancer cell lines (6).

Transforming acid coiled-coil (TACC) proteins are a family of proteins characterized by a common coiled-coil motif of approximately 200 amino acids at the carboxy-terminal end (7). When phosphorylated at Ser558 by Aurora A, mammalian TACC3 is localized to mitotic spindles and increases microtubule stability (8,9).

Aurora A-dependent phosphorylation of CENP-A on Ser7 during prophase is required for proper targeting of Aurora B to the inner centromere in prometaphase, proper kinetochore/microtubule attachment and proper alignment of chromosomes during mitosis (10). Aurora B also targets Ser7 on CENP-A, which in turn regulates Aurora B activity during cytokinesis (11). Aurora B phosphorylates both Ser10 and Ser28 on histone H3 in concordance with mitotic chromosome condensation (12).

Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (13). Aurora A phosphorylates p53 at Ser315 in a cell cycle-dependent manner leading to MDM2-mediated ubiquitination/degradation of p53 (14). Aurora A phosphorylation of Thr210 on PLK promotes mitotic entry following checkpoint-dependent cell cycle arrest (15).

1.  Warner, S.L. et al. (2003) Mol Cancer Ther 2, 589-95.

2.  Katayama, H. et al. (2003) Cancer Metastasis Rev 22, 451-64.

3.  Andrews, P.D. et al. (2003) Curr Opin Cell Biol 15, 672-83.

4.  Pascreau, G. et al. (2003) Prog Cell Cycle Res 5, 369-74.

5.  Crosio, C. et al. (2002) Mol Cell Biol 22, 874-85.

6.  Kimura, M. et al. (1999) J Biol Chem 274, 7334-40.

7.  Gergely, F. et al. (2000) Proc Natl Acad Sci U S A 97, 14352-7.

8.  Levine, A.J. (1997) Cell 88, 323-31.

9.  Kinoshita, K. et al. (2005) J Cell Biol 170, 1047-55.

10.  Schneider, L. et al. (2007) J Biol Chem 282, 29273-83.

11.  Kunitoku, N. et al. (2003) Dev Cell 5, 853-64.

12.  Zeitlin, S.G. et al. (2001) J Cell Biol 155, 1147-57.

13.  Goto, H. et al. (2002) Genes Cells 7, 11-7.

14.  Macůrek, L. et al. (2008) Nature 455, 119-23.

15.  Sakaguchi, K. et al. (1998) Genes Dev 12, 2831-41.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.

Aurora A/B Substrate Antibody Sampler Kit