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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Beclin-1 (D40C5) Rabbit mAb 3495 20 µl
Western Blotting Immunoprecipitation
H M R Mk 60 Rabbit IgG
LC3A/B (D3U4C) XP® Rabbit mAb 12741 20 µl
Western Blotting Immunohistochemistry Immunofluorescence Flow Cytometry
H M R 14, 16 Rabbit IgG
Atg5 (D5F5U) Rabbit mAb 12994 20 µl
Western Blotting Immunoprecipitation
H M R 55 Rabbit IgG
Atg12 (D88H11) Rabbit mAb 4180 20 µl
Western Blotting Immunoprecipitation
H M R Mk 16, 55 Rabbit IgG
Atg16L1 (D6D5) Rabbit mAb 8089 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R 66, 68 Rabbit IgG
Atg7 (D12B11) Rabbit mAb 8558 20 µl
Western Blotting Immunoprecipitation
H M R 78 Rabbit IgG
Atg3 Antibody 3415 20 µl
Western Blotting
H M R Mk 40 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting

Product Description

The Autophagy Antibody Sampler Kit provides an economical means to investigate the molecular machinery of autophagy within the cell. The kit contains enough primary and secondary antibodies to perform two Western blot experiments.

Specificity / Sensitivity

Each antibody in the Autophagy Antibody Sampler Kit detects its respective target at endogenous levels. Both the Atg5 and Atg12 rabbit monoclonal antibodies recognize the Atg12-Atg5 conjugate.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues surrounding Leu44 of human LC3B, Thr72 of human Beclin-1, Leu265 of human Atg5, Ser36 of human Atg12, or near the amino termini of human Atg7 and Atg16L1. Polyclonal antibodies are produced by immunizing animals with synthetic peptides corresponding to residues near the amino-termini of human Atg3. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

1.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot. Cell 1, 11-21.

2.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ. 12 Suppl 2, 1509-18.

3.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

4.  Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.

5.  Mizushima, N. et al. (1998) Nature 395, 395-8.

6.  Suzuki, K. et al. (2001) EMBO J 20, 5971-81.

7.  Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.

8.  Shintani, T. et al. (1999) EMBO J 18, 5234-41.

Entrez-Gene Id 9140 , 55054 , 64422 , 9474 , 10533 , 8678 , 84557 , 81631
Swiss-Prot Acc. O94817 , Q676U5 , Q9NT62 , Q9H1Y0 , O95352 , Q14457 , Q9H492 , Q9GZQ8

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Autophagy Antibody Sampler Kit