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Santa Cruz discontinued a large number of its polyclonal products as a result of the USDA settlement that was made public May 19th 2016

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32345T 1 Kit (4 x 20 µl) $299.00.0
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Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Atg5 (D5F5U) Rabbit mAb 12994 20 µl
Western Blotting Immunoprecipitation
H M R 55 Rabbit IgG
Atg12 (D88H11) Rabbit mAb 4180 20 µl
Western Blotting Immunoprecipitation
H M R Mk 16, 55 Rabbit IgG
Atg16L1 (D6D5) Rabbit mAb 8089 20 µl
Western Blotting Immunoprecipitation Immunofluorescence
H M R 66, 68 Rabbit IgG
Atg7 (D12B11) Rabbit mAb 8558 20 µl
Western Blotting Immunoprecipitation
H M R 78 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
Western Blotting
Goat 

Product Description

The Autophagy Vesicle Elongation (Atg12 Conjugation) Antibody Sampler Kit provides an economical means of detecting proteins related to autophagy vesicle elongation pathway. The kit contains enough antibody to perform two western blot experiments per primary antibody.


Specificity / Sensitivity

Atg5 (D5F5U) Rabbit mAb recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 conjugated to Atg12. Atg12 (D88H11) Rabbit mAb detects endogenous levels of total free and Atg5 bound Atg12 protein. Atg16L1 (D6D5) Rabbit mAb recognizes endogenous levels of total Atg16L1 protein. A background band is detected at 40 kDa in some cell lines. Atg7 (D12B11) Rabbit mAb recognizes endogenous levels of total Atg7 protein.


Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu265 of human Atg5 protein, a synthetic peptide corresponding to residues surrounding Ser36 of human Atg12 protein, a synthetic peptide corresponding to residues near the amino terminus of human Atg16L1 protein, and a synthetic peptide corresponding to residues near the amino terminus of human Atg7 protein.

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8). Atg16L1 binds Atg5 of the Atg12-Atg5 conjugate forming an 800 kDa multimeric complex (9). The Atg12-Atg-5-Atg16L1 complex localizes to pre-autophagosomal membranes where it determines the site of LC3 lipidation and catalyzes the reaction required for the formation of mature autophagosomes (9,10).


1.  Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.

2.  Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.

3.  Levine, B. and Yuan, J. (2005) J. Clin. Invest. 115, 2679-88.

4.  Mizushima, N. et al. (2003) J Cell Sci 116, 1679-88.

5.  Mizushima, N. et al. (1998) J Biol Chem 273, 33889-92.

6.  Mizushima, N. et al. (1998) Nature 395, 395-8.

7.  Fujita, N. et al. (2008) Mol Biol Cell 19, 2092-100.

8.  Suzuki, K. et al. (2001) EMBO J 20, 5971-81.

9.  Tanida, I. et al. (1999) Mol Biol Cell 10, 1367-79.

10.  Shintani, T. et al. (1999) EMBO J 18, 5234-41.


Entrez-Gene Id 9140 , 55054 , 9474 , 10533
Swiss-Prot Acc. O94817 , Q676U5 , Q9H1Y0 , O95352


For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.

32345
Autophagy Vesicle Elongation (Atg12 Conjugation) Antibody Sampler Kit