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4977
Axl Antibody
Primary Antibodies

Axl Antibody #4977

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Citations (6)

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# Product Name Application Reactivity
  • WB
H Mk
  • WB
  • IP
  • F
H Mk
  • WB
H Mk Pg
  • WB
  • IP
  • IHC
  • IF
  • F
H Mk

Supporting Data

REACTIVITY
SENSITIVITY
MW (kDa) 138
SOURCE Rabbit

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

Axl Antibody detects endogenous levels of Axl protein. The antibody does not cross-react with related proteins.

Species predicted to react based on 100% sequence homology:

Bovine

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to sequences surrounding amino acid residue 740 of human Axl. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Axl, Sky, and Mer are three members of a receptor tyrosine kinase (RTK) family that share a conserved intracellular tyrosine kinase domain and an extracellular domain similar to those seen in cell adhesion molecules. These RTKs bind the vitamin K-dependent protein growth-arrest-specific 6 (Gas6), which is structurally related to the protein S anticoagulation factor (1). Upon binding to its receptor, Gas6 activates phosphatidylinositol 3-kinase (PI3K) and its downstream targets Akt and S6K, as well as NF-κB (2,3). A large body of evidence supports a role for Gas6/Axl signaling in cell growth and survival in normal and cancer cells (4).

  1. Crosier, K.E. and Crosier, P.S. (1997) Pathology 29, 131-5.
  2. Demarchi, F. et al. (2001) J Biol Chem 276, 31738-44.
  3. Lee, W.P. et al. (2002) Oncogene 21, 329-36.
  4. Bellosta, P. et al. (1997) Oncogene 15, 2387-97.

Pathways & Proteins

Explore pathways + proteins related to this product.

For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

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