Cat. # | Size | Qty. | Price |
---|---|---|---|
2951T | 1 Kit (5 x 20 microliters) |
|
Product Includes | Quantity | Applications | Reactivity | MW(kDa) | Isotype |
---|---|---|---|---|---|
β-Catenin (D10A8) XP® Rabbit mAb 8480 | 20 µl |
|
H M R Mk | 92 | Rabbit IgG |
Phospho-β-Catenin (Ser33/37/Thr41) Antibody 9561 | 20 µl |
|
H M R Mk | 92 | Rabbit |
Phospho-β-Catenin (Thr41/Ser45) Antibody 9565 | 20 µl |
|
H M Mk | 92 | Rabbit |
Phospho-β-Catenin (Ser552) (D8E11) Rabbit mAb 5651 | 20 µl |
|
H M | 92 | Rabbit IgG |
Phospho-β-Catenin (Ser675) (D2F1) XP® Rabbit mAb 4176 | 20 µl |
|
H M R | 92 | Rabbit IgG |
Anti-rabbit IgG, HRP-linked Antibody 7074 | 100 µl |
|
Goat |
Product Information
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser33, Ser37 and Thr41 of human β-catenin or residues surrounding Thr41 and Ser45 of human β-catenin. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein, a synthetic phosphopeptide corresponding to residues surrounding Ser552 of human β-catenin protein, or a synthetic phosphopeptide corresponding to residues surrounding Ser675 of human β-catenin.
β-catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
Both Akt and PKA were shown to phosphorylate β-catenin at Ser552 and Ser675. Phosphorylation at Ser552 and Ser675 induces β-catenin accumulation in the nucleus and increases its transcriptional activity (9-12).
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