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84441
β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free) #84441

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  1. WB
  2. IHC
  3. IF
Western Blotting Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Western Blotting Image 2: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from control HeLa cells (lane 1) or HeLa cells with an apparent in-frame deletion in the gene encoding β-Catenin (lane 2), using β-Catenin (D10A8) XP® Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). The change in molecular weight in the mutated HeLa cells is consistent with an in-frame deletion. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using ß-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb. Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free)
Confocal immunofluorescent analysis of HeLa (left, positive) and NCI-H28 (right, negative) cells using β-Catenin (D10A8) XP®Rabbit mAb (green) and DRAQ5® #4084 (fluorescent DNA dye) (blue). Data were generated using the standard formulation of this product.
To Purchase # 84441SF
Product # Size Price
84441SF
100 µg $ 746

Supporting Data

REACTIVITY H M R Mk
SENSITIVITY Endogenous
MW (kDa) 92
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #8480. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #8480

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

β-Catenin (D10A8) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total β-catenin protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology:

Zebrafish, Bovine, Pig, Horse, Guinea Pig

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro714 of human β-catenin protein.

Background

β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines (8).
  1. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-3305.
  2. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88.
  3. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21.
  4. Amit, S. et al. (2002) Genes Dev 16, 1066-76.
  5. Liu, C. et al. (2002) Cell 108, 837-47.
  6. Yanagawa, S. et al. (2002) EMBO J 21, 1733-42.
  7. Yost, C. et al. (1996) Genes Dev 10, 1443-54.
  8. Morin, P.J. et al. (1997) Science 275, 1787-90.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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