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58798
B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Primary Antibodies
Monoclonal Antibody

B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free) #58798

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  1. WB
  2. IHC
Western Blotting Image 1: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from various cell lines using B7-H3 (D9M2L) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data was generated using the standard formulation of this product.
Western Blotting Image 2: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Western blot analysis of extracts from COS-7 cells, mock transfected (-) or transfected with constructs expressing Myc-tagged full-length human PD-L1, PD-L2, B7-H3, or B7-H4 protein (as indicated), using B7-H3 (D9M2L) XP® Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 1: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded colon carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 2: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded lung carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
Immunohistochemistry Image 3: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded ovarian carcinoma using B7-H3 (D9M2L) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 4: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Multiplex immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using B7-H3 (D9M2L) XP® rabbit mAb (red), PD-1 (D4W2J) XP® rabbit mAb #86163 (green), B7-H4 (D1M8I) XP® rabbit mAb #14572 (cyan), LAG3 (D2G4O) XP® rabbit mAb #15372 (magenta), TIM-3 (D5D5R) XP® rabbit mAb #45208 (yellow), and VISTA (D1L2G) XP® rabbit mAb #64953 (orange). Data was generated using the standard formulation of this product.
Immunohistochemistry Image 5: B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded LNCaP (left) and NCI-H69 (right) cell pellets using B7-H3 (D9M2L) XP® Rabbit mAb. Data was generated using the standard formulation of this product.
To Purchase # 58798SF
Product # Size Price
58798SF
100 µg $ 746

Supporting Data

REACTIVITY H
SENSITIVITY Endogenous
MW (kDa) 90
Source/Isotype Rabbit IgG

Application Key:

  • WB-Western Blot
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Vir-Virus
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

This product is the carrier free version of product #14058. All data was generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. Optimal dilutions/concentrations should be determined by the end user.

Formulation

Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #14058

Storage

Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

B7-H3 (D9M2L) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total B7-H3 protein.

Species Reactivity:

Human

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala94 of human B7-H3 protein.

Background

B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8).
  1. Chapoval, A.I. et al. (2001) Nat Immunol 2, 269-74.
  2. Leitner, J. et al. (2009) Eur J Immunol 39, 1754-64.
  3. Ling, V. et al. (2003) Genomics 82, 365-77.
  4. Petroff, M.G. et al. (2005) Am J Pathol 167, 465-73.
  5. Roth, T.J. et al. (2007) Cancer Res 67, 7893-900.
  6. Loo, D. et al. (2012) Clin Cancer Res 18, 3834-45.
  7. Seaman, S. et al. (2007) Cancer Cell 11, 539-54.
  8. Zang, X. et al. (2010) Mod Pathol 23, 1104-12.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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