B7-H3 (D9M2L) XP® Rabbit mAb #14058
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #58798.
Protocol
Specificity / Sensitivity
B7-H3 (D9M2L) XP® Rabbit mAb recognizes endogenous levels of total B7-H3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala94 of human B7-H3 protein.
Background
B7 homolog 3 (B7-H3, CD276) is a member of the B7 family of cell surface ligands that regulate T cell activation and immune responses. B7-H3 protein contains two extracellular Ig-like V-type domains and two IgG-like C2-type domains, a transmembrane domain, and a short intracellular domain (1,2). Early research examining the biological process of B7-H3 suggested that B7-H3 is a positive regulator of T cell response (1). Subsequent research studies indicated that B7-H3 is a negative regulator of T cell response, and that the protein inhibits T cell proliferation (2,3). One possibility is that B7-H3 interacts with two distinct sets of receptors, resulting in seemingly opposite biological outcomes (2). B7-H3 is expressed by antigen presenting cells, activated T cells, and a few normal tissues, including placenta and prostate (1,4,5). Expression of B7-H3 is seen in several cancer types, including prostate, breast, colon, lung, and gastric cancers, and in endothelial cells from tumor associated vasculature (6-8).
- Chapoval, A.I. et al. (2001) Nat Immunol 2, 269-74.
- Leitner, J. et al. (2009) Eur J Immunol 39, 1754-64.
- Ling, V. et al. (2003) Genomics 82, 365-77.
- Petroff, M.G. et al. (2005) Am J Pathol 167, 465-73.
- Roth, T.J. et al. (2007) Cancer Res 67, 7893-900.
- Loo, D. et al. (2012) Clin Cancer Res 18, 3834-45.
- Seaman, S. et al. (2007) Cancer Cell 11, 539-54.
- Zang, X. et al. (2010) Mod Pathol 23, 1104-12.
Limited Uses
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