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8223S 1 Kit (10 western blots) $472.00.0


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PhosphoSitePlus® Resource

  • Additional protein information
  • Analytical tools


Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Phospho-Bad (Ser112) (40A9) Rabbit mAb 5284 100 µl
Western Blotting Immunohistochemistry Flow Cytometry
H M R Mk 23 Rabbit IgG
Bad (D24A9) Rabbit mAb 9239 100 µl
Western Blotting
H M R Mk 23 Rabbit IgG

Product Usage Information

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

1.  Yang, E. et al. (1995) Cell 80, 285-291.

2.  Zha, J. et al. (1996) Cell 87, 619-628.

3.  Datta, S.R. et al. (1997) Cell 91, 231-241.

4.  Peso, L. et al. (1997) Science 278, 687-689.

5.  Bonni, A. et al. (1999) Science 286, 1358-1362.

6.  Tan, Y. et al. (1999) J. Biol. Chem. 274, 34859-34867.

7.  Harada, H. et al. (1999) Mol. Cell 3, 413-422.

8.  Tan, Y. et al. (2000) J. Biol. Chem. 275, 25865-25869.

9.  Lizcano, J. et al. (2000) Biochem. J. 349, 547-557.

10.  Datta, S. et al. (2000) Mol. Cell 6, 41-51.

Entrez-Gene Id 572
Swiss-Prot Acc. Q92934

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

PhosphoPlus® Bad (Ser112) Antibody Duet