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66986
PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet
Primary Antibodies
Antibody Duet

PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet #66986

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PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet: Image 1

Immunoprecipitation of BCKDH-E1α from MCF7 extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is BCKDH-E1α (E4T3D) Rabbit mAb. Western blot analysis was performed using BCKDH-E1α (E4T3D) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet: Image 2

Immunoprecipitation of Phospho-BCKDH-E1α (Ser293) from 293T extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb. Western blot analysis was performed using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as the secondary antibody.

PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet: Image 3

Western blot analysis of extracts from human liver, MCF7 and PANC-1 cell lines using BCKDH-E1α (E4T3D) Rabbit mAb.

PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet: Image 4

Western blot analysis of extracts from various cell lines using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb.

PhosphoPlus® BCKDH-E1α (Ser293) Antibody Duet: Image 5

Western blot analysis of extracts from HeLa cells, untreated (-) or treated (+) with calf intestinal alkaline phosphatase (CIP)/λ phosphatase, using Phospho-BCKDH-E1α (Ser293) (E2V6B) Rabbit mAb (upper) or BCKDH-E1α (E4T3D) Rabbit mAb #90198 (lower).

To Purchase # 66986S
Product # Size Price
66986S
1 Kit $ 514

Product Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5).

Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH (3,4). A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice (5).

  1. Li, T. et al. (2017) Cell Metab 25, 374-85.
  2. Shin, A.C. et al. (2014) Cell Metab 20, 898-909.
  3. Lu, G. et al. (2009) J Clin Invest 119, 1678-87.
  4. Harris, R.A. et al. (1997) Adv Enzyme Regul 37, 271-93.
  5. Lian, K. et al. (2015) Diabetes 64, 49-59.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
PhosphoPlus is a trademark of Cell Signaling Technology, Inc.