Western blot analysis of extracts from various cell lines using BCL7A Antibody.
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
BCL7A Antibody recognizes endogenous levels of total BCL7A protein. This antibody does not cross-react with other BCL7 proteins.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val168 of human BCL7A protein. Antibodies are purified by peptide affinity chromatography.
The BCL7 family of proteins consisting of BCL7A, BCL7B, and BCL7C are part of the mammalian SWI/SNF complex (1,2). The BCL7 family has tumor suppressive properties, and differential expression is correlated with poor prognosis of multiple cancer types (2-6). BCL7A was first identified as gene invoked in a MYC-BCL7A fusion in high-grade B-cell non-Hodgkin lymphoma (7). BCL7A also contributes to the function of Purkinje cells (8). BCL7B is deleted in Williams-Beuren syndrome and has been found to play a role in Wnt signaling (9,10).
Explore pathways + proteins related to this product.
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