BLK (E8T1B) Rabbit mAb #66002
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
BLK (E8T1B) Rabbit mAb recognizes endogenous levels of total BLK protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro228 of human BLK protein.
Background
BLK is a Src family protein tyrosine kinase expressed in all stages of B cell development (1,2). Activation of B cells by various ligands is accompanied by activation of BLK (3). It has been suggested that BLK is involved in the control of B cell differentiation and proliferation (4,5). BLK transcripts have also been detected in human thymocytes, but not in mature T cells, implicating that BLK may play an important role in thymopoiesis (6). BLK function may be redundant, however, as mice that do not express BLK are not impaired with respect to B cell development and immune response (7).
- Dymecki, S.M. et al. (1990) Science 247, 332-336.
- Wasserman, R. et al. (1995) J. Immunol. 155, 644-651.
- Sefton, B.M. et al. (1994) Curr. Opin. Immunol. 6, 372-379.
- Malek, S.N. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 7351-7356.
- Yao, X.R. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 7946-7950.
- Islam, K.B. et al. (1995) J. Immunol. 154, 1265-1272.
- Texido, G. et al. (2000) Mol Cell Biol 20, 1227-33.
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