Western blot analysis of extracts from various cell lines using BOB-1/OBF-1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, BOB-1/OBF-1 protein is not detected in C2C12 cells.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with Myc/DDK-tagged hBOB-1/OFB-1 (+) using BOB-1/OBF-1 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using BOB-1/OBF-1 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, BOB-1/OBF-1 protein is not detected in HDLM-2 cells.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
BOB-1/OBF-1 Antibody recognizes endogenous levels of total BOB-1/OBF-1 protein.
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro209 of human BOB-1/OBF-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
B-cell Oct binding factor-1 (BOB-1/OBF-1) is a B-cell restricted transcriptional coactivator. BOB-1 facilitates transactivation of immunoglobulins and other B-cell specific genes through the binding and activation of the transcription factors Oct-1 and Oct-2 (1-4). Research studies have demonstrated that BOB-1 expression is required for antigen-dependent B-cell maturation (5-7). In pathological conditions such as classical Hodgkin’s disease, loss of BOB-1 expression is thought, in part, to contribute to the defect in immunoglobulin gene expression by Hodgkin and Reed Sternberg cells (8,9). In the context of multiple myeloma, overexpression of BOB-1 has been shown to contribute to malignant plasma cell cell growth, in part, through enhanced transactivation of TNFRSF17/BCMA (10).
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