BRDT (E6T6E) Rabbit mAb #93069
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 140 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
BRDT (E6T6E) Rabbit mAb recognizes endogenous levels of total BRDT protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human BRDT protein.
Background
Testis-specific bromodomain-containing protein (BRDT) is a member of the bromodomains and extra terminal (BET) family of proteins, which also includes BRD2, BRD3, and BRD4 (1-3). BET family proteins contain two tandem bromodomains and an extra terminal (ET) domain, and bind acetyl lysine residues (3). BRDT is expressed in the testes and is essential for male germ cell differentiation, acting as a master regulator of meiotic divisions and post-meiotic genome repackaging (2,4). JQ1 treatment of BRDT has been explored as a potential male contraceptive, as JQ1-treated mice exhibit reduced spermatozoa number and motility without affecting hormone levels (5).
Limited Uses
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