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Render Timestamp: 2024-12-09T10:51:21.634Z
Commit: 224419269841c11382c4555dbee545259bf6c379
XML generation date: 2024-09-12 14:39:19.531
Product last modified at: 2024-10-24T19:00:08.327Z
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PDP - Template Name: Antibody Sampler Kit
PDP - Template ID: *******4a3ef3a

c-Kit Antibody Sampler Kit #9370

    Product Information

    Product Description

    The c-Kit Antibody Sampler Kit provides a fast and economical means of evaluating levels of c-Kit receptor protein phosphorylated at the specified sites, as well as total c-Kit receptor levels. The kit contains enough primary and secondary antibody to perform two Western blot experiments.

    Specificity / Sensitivity

    Phospho-c-Kit antibodies detect endogenous levels of c-Kit when phosphorylated at the specified sites. c-Kit (D13A2) XP® Rabbit mAb detects endogenous levels of total c-kit receptor protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Tyr703 of human c-Kit. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

    Background

    c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types, including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components, including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).
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