Western blot analysis of extracts from MKN-45 cells treated with λ-phosphatase or Forskolin #3828 (30 μM, 20 min) using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb (upper) and CaMKII-α (D10C11) Rabbit mAb #11945 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (lane 1) or expressing CaMKII-α (lane2), CaMKII-β (lane 3) or CaMKII-γ (lane 4) using CaMKII (pan) (D11A10) Rabbit mAb.
Western blot analysis of extracts from rat brain, mouse brain and human cerebellum using Phospho-CaMKII (Thr286) (D21E4) Rabbit mAb.
Western blot analysis of extracts from mouse and rat brain using CaMKII (pan) (D11A10) Rabbit mAb.
Western blot analysis of extracts from 293T cells, untransfected or transfected with specific CamKII proteins as indicated, using Phospho-CamKII (Thr286) (D21E4) Rabbit mAb. Antibody phosphospecificity was verified by preincubating the antibody in the absence of a peptide (-) or with either CamKII-β (Thr287) phosphopeptide (+) or CamKII-β (Thr287) non-phosphopeptide (+) prior to incubating the membrane.
PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. Ca2+/calmodulin binding to the CaMKII regulatory domain relieves autoinhibition and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents phospho-CaMKII (Thr286) dephosphorylation through an inhibitory effect on PP1 (4).
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