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Caspase-10 Antibody #9752

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  • WB

Inquiry Info. # 9752

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    Product Specifications

    REACTIVITY H M R
    SENSITIVITY Endogenous
    MW (kDa) 63 to 66
    SOURCE Rabbit
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 
    • R-Rat 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Caspase-10 Antibody detects endogenous levels of full length caspase-10 and its various isoforms. This antibody does not cross-react with other caspases.

    Species Reactivity:

    Human, Mouse, Rat

    Source / Purification

    Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding aspartic acid 219 of human caspase-10. Antibodies are purified by protein A and peptide affinity chromatography.

    Background

    Caspase-10 is a DED (death effector domain)-containing caspase and functions as an initiator caspase in Fas/TNF induced apoptosis (1). Four isoforms of caspase-10 have been identified: caspase-10a (Mch4), caspase-10b (FLICE2), caspase-10c and caspase-10d. They have the same prodomain but different mature large and small subdomains (2-4). Upon death ligand-receptor binding, caspase-10 is coupled to the multimeric Fas/TNF receptor complex via DED/FADD adaptor interaction (1-4). This complex processes procaspase-10 into a large active fragment and a small fragment. Cleaved caspase-10 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, leading to apoptosis (3-6).

    Alternate Names

    ALPS2; Apoptotic protease Mch-4; CASP-10; CASP10; CASPA; caspase 10; caspase 10 apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine peptidase; caspase 10, apoptosis-related cysteine protease; Caspase-10; Caspase-10 subunit p12; Caspase-10 subunit p23/17; FADD-like ICE2; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE-2; FLICE2; ICE-like apoptotic protease 4; interleukin-1B-converting enzyme 2; MCH4

    For Research Use Only. Not for Use in Diagnostic Procedures.
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