Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma in situ using Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded cell pellets, caspase-3 positive HeLa (left) and Caspase-3 negative MCF7 (right), using Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated).
Immunohistochemical analysis of paraffin-embedded human tonsil using Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|DETECTION REAGENT/SUBSTRATE COMPATIBILITY|
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised April 2020
Protocol Id: 283
Caspase-3 (D3R6Y) Rabbit mAb (IHC Formulated) recognizes endogenous levels of total caspase-3 protein by immunohistochemistry.Species Reactivity:
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the p20 subunit of human caspase-3 protein.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
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SignalStain is a trademark of Cell Signaling Technology, Inc.